CPA1 Antikörper (HRP)

Produktnummer ABIN7565867

Der Kaninchen Polyklonal Anti-CPA1-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, CPA1 in Proben von Rind (Kuh) zu detektieren.

Kurzübersicht für CPA1 Antikörper (HRP) (ABIN7565867)

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CPA1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-CPA1 Antikörper (HRP)

Verwendungszweck: Carboxypeptidase A Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].

Produktmerkmale: Synonyms: rabbit anti-Carboxypeptidase A antibody peroxidase conjugation, HRP conjugated rabbit anti-Carboxypeptidase A Antibody, Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody

Aufreinigung: Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Carboxypeptidase A [Bovine Pancreas]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Carboxypeptidase A Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:300 - 1:2,000 ELISA Dilution: 1:3,000 - 1:10,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CPA1

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Andere Bezeichnung: CPA1

Hintergrund: Background: Carboxypeptidase A (CPA-1) is produced in the pancreas and is crucial to many processes in the human body to include digestion, post-translational modification of proteins, blood clotting, and reproduction. There are two proposed mechanisms for the catalytic function of carboxypeptidase A. The first is a nucleophilic pathway involving a covalent acyl enzyme intermediate containing active site base Glu-270. The second proposed mechanism is a promoted water pathway. This mechanism involves attack of a water molecule at the scissile peptide linkage of the substrate.

Gen-ID: 286762

NCBI Accession: NP_777175

UniProt: P00730