CA2 Antikörper (HRP)

Produktnummer ABIN7565866

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CA2 in WB, IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CA2 Antikörper (HRP) (ABIN7565866)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CA2 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-CA2 Antikörper (HRP)

Verwendungszweck: Carbonic Anhydrase II Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes].

Produktmerkmale: Synonyms: rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II

Aufreinigung: Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Carbonic Anhydrase II [Human Erythrocytes]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Carbonic Anhydrase II Peroxidase Antibody has been tested by Western blot and suitable to be assayed against 1.0 μg of Carbonic Anhydrase II [Human Erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:100 - 1:500 Western Blot Dilution: 1:250 - 1:1,000 ELISA Dilution: 1:500 - 1:2,500 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Andere Bezeichnung: CA2

Hintergrund: Background: Carbonic anhydrase II (CA2), is one of fourteen forms of human α carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

Gen-ID: 760

NCBI Accession: NP_000058

UniProt: P00918