CA1 Antikörper (HRP)

Produktnummer ABIN7565864

Der Ziege Polyklonal Anti-CA1-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, CA1 in Proben von Human zu detektieren.

Kurzübersicht für CA1 Antikörper (HRP) (ABIN7565864)

Target: CA1 (Carbonic Anhydrase I (CA1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CA1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-CA1 Antikörper (HRP)

Verwendungszweck: Carbonic Anhydrase I Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ].

Produktmerkmale: Synonyms: goat anti-Carbonic Anhydrase I Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Carbonic Anhydrase I Antibody, Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody

Aufreinigung: Carbonic Anhydrase I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Carbonic Anhydrase I [Human Erythrocytes ]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Carbonic Anhydrase I peroxidase is tested by ELISA and western blot and useful in immunohistochemistry. Optimal titers should be obtained by researchers. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CA1

Target: CA1 (Carbonic Anhydrase I (CA1))

Andere Bezeichnung: CA1

Hintergrund: Background: Carbonic Anhydrase I is an enzyme that in humans is encoded by the CA1 gene. Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.

Gen-ID: 759

NCBI Accession: NP_001122301

UniProt: P00915