BLRO_MYRVE Antikörper (HRP)

Produktnummer ABIN7565861

Der Ziege Polyklonal anti-BLRO_MYRVE Antikörper wird verwendet zum Nachweis von BLRO_MYRVE in Proben von Myrothecium verrucaria. Er wurde validiert für ELISA, IP und WB.

Kurzübersicht für BLRO_MYRVE Antikörper (HRP) (ABIN7565861)

Target: BLRO_MYRVE

Reaktivität: Myrothecium verrucaria

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Produktdetails

Verwendungszweck: Bilirubin Oxidase Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Bilirubin Oxidase [Myrothecium verrucaria].

Produktmerkmale: Synonyms: goat anti-Bilirubin Oxidase HRP conjugation, goat anti-Bilirubin Oxidase Antibody Peroxidase Conjugated

Aufreinigung: Bilirubin Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Bilirubin Oxidase [Myrothecium verrucaria]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Bilirubin Oxidase has been tested by western blot and is suitable to be assayed against 1.0 μg of Bilirubin Oxidase [Myrothecium verrucaria] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:2,500 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: BLRO_MYRVE

Hintergrund: Background: Bilirubin Oxidase is key in the oxidation of bilirubin and other tetrapyrroles. This enzyme belongs to the family of oxidoreductases, to be specific those acting on the CH-CH group of donor with oxygen as acceptor. The systematic name of this enzyme class is bilirubin:oxygen oxidoreductase. This enzyme is also called bilirubin oxidase M-1. This enzyme participates in porphyrin and chlorophyll metabolism.

UniProt: Q12737