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beta-Amylase 1 Antikörper (HRP)

Der Kaninchen Polyklonal Anti-beta-Amylase 1-Antikörper wurde für WB, ELISA und DB validiert. Er ist geeignet, beta-Amylase 1 in Proben von Sweet potato zu detektieren.
Produktnummer ABIN7565858

Kurzübersicht für beta-Amylase 1 Antikörper (HRP) (ABIN7565858)

Target

beta-Amylase 1 (BAM1)

Reaktivität

  • 4
  • 1
Sweet potato

Wirt

  • 5
Kaninchen

Klonalität

  • 5
Polyklonal

Konjugat

  • 3
  • 1
  • 1
Dieser beta-Amylase 1 Antikörper ist konjugiert mit HRP

Applikation

  • 5
  • 4
  • 2
  • 1
Western Blotting (WB), ELISA, Dot Blot (DB)
  • Verwendungszweck

    Beta Amylase Antibody Peroxidase Conjugated

    Kreuzreaktivität (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Beta Amylase [Sweet Potato].

    Produktmerkmale

    Synonyms: rabbit anti-Beta Amylase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Beta Amylase Antibody, 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody

    Aufreinigung

    Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: Beta Amylase [Sweet Potato]

    Immunogen Type: Native Protein

  • Applikationshinweise

    Application Note: Anti-Beta Amylase Peroxidase conjugated antibody has been tested by ELISA and dot blot This product is assayed against 1.0 μg of Beta Amylase [Sweet Potato] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:250 Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target

    beta-Amylase 1 (BAM1)

    Andere Bezeichnung

    BMY1, AMYB

    Hintergrund

    Background: Beta Amylase acts on starch, glycogen and related polysaccharides and oligosaccharides producing beta-maltose by an inversion.

    UniProt

    P10537
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