Protein G Antikörper (FITC)

Produktnummer ABIN7565828

Produktdetails anti-Protein G Antikörper (FITC)

Target: Protein G

Reaktivität: Streptococcus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Protein G Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Dot Blot (DB), Western Blotting (WB), Fluorescence Microscopy (FM), FLISA

Verwendungszweck: Protein G Antibody Fluorescein Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Protein G.

Produktmerkmale: Synonyms: rabbit anti-Protein G Antibody FITC Conjugation, Fluorescein conjugated rabbit anti-Protein G Antibody, Protein G FITC

Aufreinigung: Anti-Protein G antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Protein G (Streptococcus species)

Immunogen Type: Native Protein

Labeling Ratio: 4.5

Anwendungsinformationen

Applikationshinweise: Application Note: FITC Conjugated Anti-Protein G antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized Western Blot Dilution: User Optimized FLISA Dilution: User Optimized IF Microscopy Dilution: 1:50 - 1:250 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Protein G

Target: Protein G

Abstract: Protein G Produkte

Hintergrund: Background: Protein G is a protein that has the property of binging to immunoglobulins. It is a 65- kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins.