PPI Antikörper (HRP)

Produktnummer ABIN7565802

Der HRP-konjugierte Ziege Polyklonal anti-PPI Antikörper (ABIN7565802) detektiert spezifisch PPI in IHC, ELISA, WB und DB. Dieser Antikörper reagiert spezifisch mit Proben aus Papaya.

Kurzübersicht für PPI Antikörper (HRP) (ABIN7565802)

Target: PPI

Reaktivität: Papaya

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Dot Blot (DB)

Produktdetails

Verwendungszweck: Papain Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Papain [Carica papaya].

Produktmerkmale: Synonyms: goat anti-Papain Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Papain Antibody, Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody

Aufreinigung: Anti-Papain is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Papain peroxidase antibody has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 μg of Papain [Carica papaya] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Researchers should determine optimal titers for applications that are not stated below. Immunohistochemistry Dilution: 1:200- 1:1,000 Western Blot Dilution: 1:500- 1:2,500 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: PPI

Hintergrund: Background: Papain Antibody detects Papain protein from papaya. Papain belongs to the peptidase C1 family and hydrolyses proteins with broad specificity for peptide bonds. The proteolysis preference of Papain is at amino acids bearing a large hydrophobic side chain at the P2 position and is inhibited when Val is at position P1. Papain may cause allergic reactions in humans. Anti-Papain Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.

UniProt: P00784