Luciferase Antikörper (FITC)

Produktnummer ABIN7565786

Der Ziege Polyklonal Anti-Luciferase-Antikörper wurde für WB, FACS, DB und FM validiert. Er ist geeignet, Luciferase in Proben von Leuchtkäfer zu detektieren.

Kurzübersicht für Luciferase Antikörper (FITC) (ABIN7565786)

Target: Luciferase

Reaktivität: Leuchtkäfer

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Luciferase Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Dot Blot (DB), Fluorescence Microscopy (FM)

Produktdetails anti-Luciferase Antikörper (FITC)

Verwendungszweck: Luciferase Antibody Fluorescein Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum and purified and partially purified Luciferase (Photinus pyralis (Firefly)).

Produktmerkmale: Synonyms: goat anti-Luciferase Antibody FITC Conjugation, Fluorescein Conjugated goat anti-Luciferase Antibody

Aufreinigung: Anti-Luciferase Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Luciferase (Photinus pyralis (Firefly))

Immunogen Type: Native Protein

Labeling Ratio: 5.6

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Luciferase Conjugated Antibody has been tested by western blot and dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized IF Microscopy Dilution: User Optimized Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Luciferase

Target: Luciferase

Hintergrund: Background: Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.