HBG1 Antikörper (N-Term)

Produktnummer ABIN7565772

Dieses Anti-HBG1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von HBG1 in ELISA und WB. Geeignet für Human.

Kurzübersicht für HBG1 Antikörper (N-Term) (ABIN7565772)

Target: HBG1 (Hemoglobin, gamma A (HBG1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HBG1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Klon: 4B3-B5-F3-B7

Produktdetails anti-HBG1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Hemoglobin beta F Antibody

Kreuzreaktivität (Details): This protein A purified mouse monoclonal antibody reacts specifically with human HbF gamma isoform.

Produktmerkmale: Synonyms: mouse anti-HbF antibody, mouse anti-hemoglobin antibody, Gamma-1-globin, Hb F Agamma, Hemoglobin gamma-1 chain, Hemoglobin gamma-A chain, HBG1, HBG2, HbF Antibody, Sickle Cell Disease (SCD)

Aufreinigung: Anti-HbF is purified from tissue culture supernatant by protein A purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Hemoglobin beta F Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Hemoglobin beta F (MOUSE) antibody has been tested ELISA and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu HBG1

Target: HBG1 (Hemoglobin, gamma A (HBG1))

Andere Bezeichnung: HBG1

Hintergrund: Background: HbF antibodies detect the hemoglobin gamma isoform subunit. Functional alternate hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 gamma subunits (alpha-2 gamma-2). Hemoglobin F is elevated in newborns, reaching adult levels by 12 months. HbF levels are increased to as much as 5 % to 10 % in normal pregnancy. Sickle cell disease (SCD), thalassemias and hemoglobinopathies occur when aberrant forms of hemoglobin are expressed in children and adults. Hemoglobin variants arise from mutations in the globin genes and sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. HbF antibody does not react other forms of Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.

Gen-ID: 3047

NCBI Accession: NP_000550

UniProt: P69891