Choline Oxidase Antikörper (HRP)

Produktnummer ABIN7565753

Der Ziege Polyklonal Anti-Choline Oxidase-Antikörper wurde für WB, ELISA und IP validiert. Er ist geeignet, Choline Oxidase in Proben von Alcaligenes zu detektieren.

Kurzübersicht für Choline Oxidase Antikörper (HRP) (ABIN7565753)

Target: Choline Oxidase

Reaktivität: Alcaligenes

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Choline Oxidase Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-Choline Oxidase Antikörper (HRP)

Verwendungszweck: Choline Oxidase Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species].

Produktmerkmale: Synonyms: goat anti-Choline Oxidase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Choline Oxidase Antibody, codA antibody

Aufreinigung: Anti-Choline oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Choline Oxidase [Alcaligenes species]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Choline oxidase has been tested by ELISA and western blot and is assayed against 1.0 μg of Choline Oxidase [Alcaligenes species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:1,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:1,000 - 1:2,500 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Choline Oxidase

Target: Choline Oxidase

Hintergrund: Background: Choline oxidase is an enzyme that catalyzes the chemical reaction (choline + O2 <=> betaine aldehyde + H2O2). Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. Choline oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycine, serine, and threonine metabolism.

Gen-ID: 116367

UniProt: P16101