CPB1 Antikörper (HRP)

Produktnummer ABIN7565752

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CPB1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Schwein.

Kurzübersicht für CPB1 Antikörper (HRP) (ABIN7565752)

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CPB1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CPB1 Antikörper (HRP)

Verwendungszweck: Carboxypeptidase B Antibody HRP Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas].

Produktmerkmale: Synonyms: rabbit anti-Carboxypeptidase B antibody HRP conjugation, peroxidase conjugated rabbit anti-Carboxypeptidase B Antibody, CarboxypeptidaseB1 antibody, CP B1 antibody, CPB 1 antibody, CPB antibody, CPB1 antibody, DKFZp779K1333 antibody, Pancreas specific protein antibody, Pancreatic carboxypeptidase B1 antibody

Aufreinigung: Anti-Carboxypeptidase B Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Carboxypeptidase B [Porcine Pancreas]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Carboxypeptidase B peroxidase Antibody has been tested in western blot and suitable to be assayed against 1.0 μg of Carboxypeptidase B [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for Anti-Carboxypeptidase B Antibody. Western Blot Dilution: 1:250 - 1:1,000 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CPB1

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Andere Bezeichnung: CPB1

Hintergrund: Background: Anti-Carboxypeptidase B Antibody is a key enzyme in the hydrolysis in basic amino acids in the C-terminal position of polypeptides. First isolated from the porcine pancreas, Carboxypeptidase B is activated by trypsin and exhibits high specificity for lysine and arginine. Carboxypeptidase B is also a key enzyme in insulin product in humans and can be used as a marker for pancreatic diagnosis. Anti-Carboxypeptidase B is ideal for investigators in Cell Biology, Immunology, and Microbiology.

Gen-ID: 397341

NCBI Accession: NP_999334

UniProt: P09955