Biotin Antikörper (FITC)

Produktnummer ABIN7565751

Der FITC-konjugierte Kaninchen Polyklonal anti-Biotin Antikörper (ABIN7565751) detektiert spezifisch Biotin in FACS und FM.

Kurzübersicht für Biotin Antikörper (FITC) (ABIN7565751)

Target: Biotin

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Biotin Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Produktdetails anti-Biotin Antikörper (FITC)

Verwendungszweck: Biotin Antibody Fluorescein Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Albumin.

Produktmerkmale: Synonyms: rabbit anti-Biotin Antibody FITC Conjugation, Fluorescein conjugated rabbit anti-Biotin antibody, Biotin FITC, Anti-Biotin fluorescein Antibody

Aufreinigung: Anti-Biotin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Biotin conjugated Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Native Protein

Labeling Ratio: 2.7

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Biotin Antibody Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized IF Microscopy Dilution: 1:50:1:300 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Biotin

Target: Biotin

Substanzklasse: Chemical

Hintergrund: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Fluorescein Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.