Avidin Antikörper (Texas Red (TR))

Produktnummer ABIN7565746

Produktdetails anti-Avidin Antikörper (Texas Red (TR))

Target: Avidin (AVD)

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Avidin Antikörper ist konjugiert mit Texas Red (TR)

Applikation: Western Blotting (WB), Dot Blot (DB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Verwendungszweck: Avidin Antibody Texas Red™ Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and anti-Avidin.

Produktmerkmale: Synonyms: rabbit anti-Avidin Antibody Texas Red™ Conjugation, Texas Red™ conjugated rabbit anti-Avidin antibody, Avidin Texas Red™, Anti-Avidin Texas Red™ Antibody, Egg White

Aufreinigung: Anti-AVIDIN Antibody Texas Red™ Conjugated was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Avidin (Hen Egg White)

Immunogen Type: Native Protein

Labeling Ratio: 3.6

Anwendungsinformationen

Applikationshinweise: Application Note: Rabbit Anti-Avidin Antibody Texas Red Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized Western Blot Dilution: >1:1,000 FLISA Dilution: >1:2,000 IF Microscopy Dilution: User Optimized Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Reconstitution Volume: 100 μL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Avidin

Target: Avidin (AVD)

Andere Bezeichnung: AVIDIN (AVD Produkte)

Hintergrund: Background: Avidin is a glycoprotein with a molecular weight of approximately 62.4 kDa. Avidin is a biotin binding protein that shows high sequence homology in birds, reptiles and amphibians. Hen egg white avidin is a tetrameric protein composed of four identical subunits, each with the ability to bind biotin with high affinity and specificity (Kd ~ 1015 M). In biotechnology, the functional consequence of tetrameric biotin binding is signal amplification. Biotin-avidin bridging is a great way to increase signal strength while maintaining specificity. The sequence of avidin only shows 30 % homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive.

UniProt: P02701