Avidin Antikörper (HRP)

Produktnummer ABIN7565744

Der Kaninchen Polyklonal Anti-Avidin-Antikörper wurde für WB, ELISA, IHC und DB validiert. Er ist geeignet, Avidin in Proben von Huhn zu detektieren.

Kurzübersicht für Avidin Antikörper (HRP) (ABIN7565744)

Target: Avidin (AVD)

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Avidin Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Produktdetails anti-Avidin Antikörper (HRP)

Verwendungszweck: Avidin Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White ].

Produktmerkmale: Synonyms: rabbit anti-Avidin Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Avidin antibody, Avidin Peroxidase, Anti-Avidin HRP Antibody, egg white

Aufreinigung: Anti-Avidin Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Avidin (Hen Egg White)

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Avidin Antibody Peroxidase Conjugated has been tested in WB and assayed against 1.0 μg of Avidin [Hen Egg White ] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:7,000 to 1:27,000 of the reconstitution concentration is suggested for this product. This product can be suitable for ELISA, Western Blot, and Immunohistochemistry. Immunohistochemistry Dilution: 1:250 - 1:1,000 Western Blot Dilution: 1:1,000 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Reconstitution Volume: 100 μL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Avidin

Target: Avidin (AVD)

Andere Bezeichnung: AVIDIN

Hintergrund: Background: Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Avidin Antibody is ideal for researchers in Immunology, Cancer, and Microbiology.

UniProt: P02701