Avidin Antikörper (FITC)

Produktnummer ABIN7565743

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Avidin in WB, DB, FACS, FLISA und FM. Er zeigt eine Reaktivität gegenüber Huhn.

Kurzübersicht für Avidin Antikörper (FITC) (ABIN7565743)

Target: Avidin (AVD)

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Avidin Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Dot Blot (DB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Produktdetails anti-Avidin Antikörper (FITC)

Verwendungszweck: Avidin Antibody Fluorescein Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Avidin.

Produktmerkmale: Synonyms: rabbit anti-Avidin Antibody fluorescein Conjugation, FITC conjugated rabbit anti-Avidin antibody, Avidin fluorescein, Anti-Avidin FITC Antibody, egg ehite

Aufreinigung: Anti-Avidin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Avidin (Hen Egg White)

Immunogen Type: Native Protein

Labeling Ratio: 3.0

Anwendungsinformationen

Applikationshinweise: Application Note: Rabbit Anti-Avidin Fluorescein conjugate has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized Western Blot Dilution: >1:1,000 FLISA Dilution: >1:1,000 IF Microscopy Dilution: User Optimized Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Avidin

Target: Avidin (AVD)

Andere Bezeichnung: AVIDIN

Hintergrund: Background: Avidin is a glycoprotein with a molecular weight of approximately 62.4 kDa. Avidin is a biotin binding protein that shows high sequence homology in birds, reptiles and amphibians. Hen egg white avidin is a tetrameric protein composed of four identical subunits, each with the ability to bind biotin with high affinity and specificity (Kd ~ 1015 M). In biotechnology, the functional consequence of tetrameric biotin binding is signal amplification. Biotin-avidin bridging is a great way to increase signal strength while maintaining specificity. The sequence of avidin only shows 30 % homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive.

UniProt: P02701