ASRGL1 Antikörper (HRP)

Produktnummer ABIN7565728

Dieses Schaf Polyklonal-Antikörper erkennt spezifisch ASRGL1 in WB und DB. Er zeigt eine Reaktivität gegenüber Rind (Kuh).

Kurzübersicht für ASRGL1 Antikörper (HRP) (ABIN7565728)

Target: ASRGL1 (Asparaginase Like 1 (ASRGL1))

Reaktivität: Rind (Kuh)

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser ASRGL1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Dot Blot (DB)

Produktdetails anti-ASRGL1 Antikörper (HRP)

Verwendungszweck: Alkaline Phosphatase Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].

Produktmerkmale: Synonyms: sheep anti-Alkaline Phosphatase HRP conjugated Antibody, peroxidase conjugated sheep anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Aufreinigung: Alkaline phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Alkaline Phosphatase [Calf Intestine]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Alkaline phosphatase peroxidase conjugated has been tested by dot blot and is suitable to be assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:250 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu ASRGL1

Target: ASRGL1 (Asparaginase Like 1 (ASRGL1))

Andere Bezeichnung: ALP1

Hintergrund: Background: Alkaline phosphatase (ALP, ALKP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65 °C inactivated most isoenzymes except Placental isoforms (PALP and SEAP).

Gen-ID: 280993

UniProt: P19111