ALDOA Antikörper (HRP)

Produktnummer ABIN7565724

Dieses Anti-ALDOA-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von ALDOA in WB, ELISA und IP. Geeignet für Kaninchen.

Kurzübersicht für ALDOA Antikörper (HRP) (ABIN7565724)

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ALDOA Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-ALDOA Antikörper (HRP)

Verwendungszweck: Aldolase Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle].

Produktmerkmale: Synonyms: goat anti-Aldolase Antibody, HRP conjugated goat anti-Aldolase Antibody, Fructose-bisphosphate aldolase A, Muscle-type aldolase, peroxidase Aldolase

Aufreinigung: Aldolase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Aldolase [Rabbit Muscle]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Aldolase has been tested by ELISA, immunoprecipitation, and western blot. This product is assayed against 1.0 μg of Aldolase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu ALDOA

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Andere Bezeichnung: ALDOA

Hintergrund: Background: Aldolase is an enzyme that performs an aldol reaction (creating an aldol) or its reverse (cleaving an aldol). Aldolase plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein. Belongs to the class I fructose-bisphosphate aldolase family.

Gen-ID: 100009055

NCBI Accession: NP_001075707

UniProt: P00883

Pathways: Ribonucleoside Biosynthetic Process