Rekombinanter HLA-E Antikörper

Produktnummer ABIN7543433

Dieses Maus Monoklonal-Antikörper erkennt spezifisch HLA-E in FACS, IF und Func. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter HLA-E Antikörper (ABIN7543433)

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HLA-E Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Klon: 3D12

Produktdetails anti-HLA-E Antikörper

Verwendungszweck: Anti-HLA-E [3D12], Mouse IgG1, kappa

Spezifität: This antibody binds an extracellular epitope on the human HLA-E, a member of human leukocyte antigen family. This antibody is capable of reacting with free HLA-E heavy chain as well as heavy chain associated with b2-microglobulin and peptide. This antibody is reported to cross react with HLA-B and HLA-C but not with HLA-A, HLA-F or HLA-G. The human HLA-E is a non-classical MHC class 1B molecule involved in immune self-nonself discrimination. HLA-E plays a very important role in cell recognition by NK cells. Peptide-bound HLA-E-B2M heterotrimeric complex primarily functions as a ligand for natural killer (NK) cell inhibitory receptor KLRD1-KLRC1, enabling NK cells to monitor the expression of other MHC class I molecules in healthy cells and to tolerate self.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing HLA-B27 transgenic mice with recombinant human HLA-E purified from AEH cells (Lymphoblastoid cell line LCL 721.221 cells transfected with HLA-E gene).

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The biotin conjugated version of this antibody was used for the study of HLA-E expression and distribution on PBMCs using flow cytometry. This antibody was also used in an assay to study the effect of HLA-E on inhibition of NKL-mediated cytolysis LCL .221 cell line. The presence of 3D11 the resulted in complete restoration of lysis (PMID: 9560253). It was reported that binding of this antibody to HLA-E is inhibited by the peptides sequences 115QFAYDGKDY123 and 137DTAAQI142. It was also reported that this antibody is capable of binding several HLA-Ia antigens like HLA-B and HLA-C (PMID: 21145594). This antibody was used in a study to determined the surface expression of HLA-E in human tumors using immunofluorescent staining and flow cytometry (PMID: 12618909).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu HLA-E

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Andere Bezeichnung: HLA-E

Hintergrund: HLAE, sHLA-E, HLA-6.2, HLA class I histocompatibility antigen alpha chain E, Major histocompatibility complex class I E, MHC, MHC class I antigen E, MHC HLA E alpha 1, MHC HLA E alpha 2.1, HLAB, HLA-B, HLA-C, HLAC

UniProt: P13747, P01889, P10321

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response