Rekombinanter Laminin Antikörper

Produktnummer ABIN7543381

Der Human Monoklonal anti-Laminin Antikörper (Klon C3_Hu10) (ABIN7543381) detektiert spezifisch Laminin in ELISA, WB, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für Rekombinanter Laminin Antikörper (ABIN7543381)

Target: Laminin (LAMA2) (Laminin, alpha 2 (LAMA2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Human

Klonalität: Monoklonal

Konjugat: Dieser Laminin Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: C3_Hu10

Produktdetails anti-Laminin Antikörper

Verwendungszweck: Anti-Laminin-2 LG5 [C3_Hu10], Human IgG1, kappa

Spezifität:

This antibody recognizes human and mouse laminin-2 LG5 and LG4/5 domain. Laminin is a large extracellular heterotrimeric glycoprotein and a major constituent of the basement membrane. It is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Application Notes original Clone: The binding specificity of this antibody for human and mouse laminin-2 LG5 was confirmed using ELISA. This antibody is capable of detecting human and mouse LG-4/5 expressed on HEK293 in a flow cytometry. This antibody can also be used for the identification recombinant human laminin-2 (Hu 211), murine LG-5 (mLG5), human LG-5 (hLG5), and human LG-4/5 (hLG4/LG5) in a western blot. This antibody was also used for the immunofluorescent staining of unfixed human and mouse muscle tissues. This antibody was used in combination with anti-laminin-2 antibody AS30_Hu6 to generate a duobody called TBTI (US11254737).

Kreuzreaktivität: Maus

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing Balb/c and Trianni mice with human laminin-2 LG5 domain. Humanized version was generated by grafting CDRs of parental mouse antibody C3 onto human framework regions.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Human

OriginalFormat: IgG1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Laminin

Target: Laminin (LAMA2) (Laminin, alpha 2 (LAMA2))

Andere Bezeichnung: Laminin-2 LG5

Hintergrund: Merosin, Laminin subunit alpha-2, LAMA2, Laminin 211, laminin G-like 5 domain, LG5, g-like 4 and 5 domain, LG-4/5, Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain, C3, humanized C3

UniProt: P24043, Q60675