Rekombinanter ITGA4 Antikörper

Produktnummer ABIN7543376

Dieses Kaninchen Chimeric-Antikörper erkennt spezifisch ITGA4 in FACS, IP, BR und InhA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter ITGA4 Antikörper (ABIN7543376)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser ITGA4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR), Inhibition Assay (InhA)

Klon: HP1-2

Produktdetails anti-ITGA4 Antikörper

Verwendungszweck: Anti-Integrin alpha 4 [HP1/2], Rabbit IgG, kappa

Spezifität: This antibody specifically binds epitope B of the alpha 4 chain of human integrin alpha 4 (CD49d). Integrins alpha-4/beta-1 (VLA-4) acts as a receptor for fibronectin and VCAM1. VLA-4 is atypical because it is mostly restricted to lymphoid and myeloid cells, and indirect evidence had suggested that it might be involved in various cell-cell interactions. In addition, VLA-4 has been shown to mediate T and B lymphocyte attachment to the heparin II binding fragment of human plasma fibronectin (FN). This antibody is also reported to cross react with integrin alpha-4/beta-7 (α4β7).

Kreuzreaktivität: Meerschweinchen

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody can be used for the immunoprecipitation of VLA-4 from cell lysates (PMID: 2430809). This antibody is also capable of detecting integrin alpha 4 expressed on the surface of Ramos cells by flow cytometry (PMID: 2430809, 1689216). This antibody can also block adherence of CTT leukocytes to recombinant soluble VCAM 1-coated plates in vitro (PMID: 7686922). This antibody can also block the interaction of VLA-4 with fibronectin. This antibody does not cause cell aggregation (PMID: 1709929). This antibody can bind soluble integrin alpha-4 in an ELISA. This antibody also inhibited the binding of PAF-stimulated eosinophils to MAdCAM-1 transfectants. This antibody also inhibited binding of unstimulated eosinophils to soluble recombinant human (rh)VCAM-1 and fibronectin (Fn) (PMID: 8911148).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Andere Bezeichnung: Integrin alpha 4

Hintergrund: CD49d, VLA-4, α4, VLA4, ITGA4, α4β1, α4 integrin, VLA4 heterodimer (α4β1), Integrin alpha-4, Integrin alpha-IV, VLA-4 subunit alpha, CD49 antigen-like family member D, B1 integrin very late activation antigen-4

UniProt: P13612

Pathways: Integrin Complex