Rekombinanter CD28 Antikörper

Produktnummer ABIN7543355

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD28 in IF, IP, ELISA und BP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter CD28 Antikörper (ABIN7543355)

Target: CD28

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD28 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Blocking Peptide (BP)

Klon: 9-3

Produktdetails anti-CD28 Antikörper

Verwendungszweck: Anti-CD28 [9.3], Mouse IgG2a, kappa

Spezifität: The antibody is specific for human CD28. CD28 is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with human T cells.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: The antibody was found to induce T cell activation and proliferation in collaboration with TPA. Further, T cell proliferation was associated with IL-2 receptor expression and IL-2 production. The antibody stained 60-80 % of the isolated T cells by immunofluorescence. The antibody immunoprecipitated CD28 from Jurkat cells. (Hara et al., 1985, PMID: 3159820). Immunofluorescence was performed on T cells expressing CD28 using this antibody. The antibody was cytotoxic for 50-80 % of peripheral T cells and for 20-50 % of thymocytes but it was not cytotoxic for cultured B-cell lines, normal B cells, or monocytes. In immune precipitation assays, the antibody reacted with CD28 (Hansen et al., 1980). The antibody induces proliferative responses in human T cells in in vitro experiments (Hutloff et al. 1999, PMID: 9930702). The antibody detected CD28 by western blot analysis (Siefken et al,1998, PMID: 9712026). Humanize, chimeric and hybrid Fab versions of the antibody were constructed. The ability of the three Fabs to bind to CD28 was examined by ELISA, the three Fabs showed a binding activity, with an EC50 of 630, 20 and 30 nM respectively for the humanized, the chimeric, hybrid Fab version. Thus, the humanized Fab showed a moderate loss in avidity. The three Fab versions of the antibody were tested for blocking ligation of CD28-Ig to CHO cells expressing CD80 and the binding was quantified by flow cytometry. The chimeric Fab exhibited the best blocking activity followed by the hybrid and the humanized Fab, with EC50 values of 0.2, 0.4, and 1.2 μM, respectively. Lastly, three Fab versions showed biological activity as an immunosuppressant by inhibiting a MLR (Tan et al. 2002, PMID: 12097421).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD28

Target: CD28

Andere Bezeichnung: CD28

Hintergrund: T-cell-specific surface glycoprotein CD28, TP44

UniProt: P10747

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response