Rekombinanter KRT14 Antikörper (AA 458-472)

Produktnummer ABIN7543316

Dieses Kaninchen Chimeric-Antikörper erkennt spezifisch KRT14 in IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter KRT14 Antikörper (AA 458-472) (ABIN7543316)

Target: KRT14 (Keratin 14 (KRT14))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser KRT14 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA

Klon: 2G4

Produktdetails anti-KRT14 Antikörper

Bindungsspezifität: AA 458-472

Verwendungszweck: Anti-Cytokeratin 14 [2G4], Rabbit IgG, kappa

Spezifität: This antibody is capable of binding human cytokeratin 14. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing 4-6 weeks old female ICR mice with recombinant protein comprising amino acids 458-472 of cytokeratin 7 in equal volume of Freund's incomplete adjuvant.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The binding characterization of this antibody was done using ELISA. The original IgG1 antibody binds cytokeratin 5/6 with a high affinity constant of 4.8x 10^(8) M^(-1). This antibody was also used in the immunohistochemical analysis of frozen paraffin sections of tonsil tissues and esophageal squamous cell carcinoma. The immunohistochemical staining pattern of this antibody is similar to commercially available antibody L1002 (CN113087793).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu KRT14

Target: KRT14 (Keratin 14 (KRT14))

Andere Bezeichnung: Cytokeratin 14

Hintergrund: CK14, CK-14, CK 14, K14, KRT14, keratin type I cytoskeletal 14, cytokeratin-14, keratin-14, CGMCC NO 20761, L1002

UniProt: P02533