Rekombinanter CD147 Antikörper

Produktnummer ABIN7543310

Dieses Kaninchen Chimeric-Antikörper erkennt spezifisch CD147 in ELISA, BP, Crys, in vivo und Inh. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter CD147 Antikörper (ABIN7543310)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD147 Antikörper ist unkonjugiert

Applikation: ELISA, Blocking Peptide (BP), Crystallization (Crys), In vivo Studies (in vivo), Inhibition (Inh)

Klon: 5F6

Produktdetails anti-CD147 Antikörper

Verwendungszweck: Anti-CD147 [5F6], Rabbit IgG, kappa

Spezifität: The antibody binds the N domain of CD147. The antibody binds to an epitope on the extracellular domain of CD147 (residues Asp65-Phe74 and Val30-Thr40). CD147 is a type I membrane glycoprotein and is classified into the immunoglobulin-like superfamily, and is expressed in many types of cancer. It is suggested that CD147 is also associated with cancer metastasis, invasion, and chemical drug resistance.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with CD147.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The specificity of the antibody was coonfirmed by competition ELISA. The antibody recognized MDA-MB-231 cells that express native CD147 antigens on tumor cell surface. To assess the ability of the original antibody to block MMP-1 production, NHLF were treated with recombinant a ECD-Flag construct and the MMP-1 production in response to CD147 stimulation was determined using the MMP-1 activity assay. The antibody showed significant inhibition of MMP-1 production. The original antibody was screened for the ability to inhibit CD147-induced MMP-2 or MMP-9 production in co-culture. Results showed the antibody inhibited MMP-2 and MMP-9 production in co-culture. The humanized version of the antibody (IgG1) inhibited CD147-stimulated MMP-1 production by fibroblast in a dose-dependent manner and the MMP-2 production by MDA-MB-231 and NHDF in a dose dependent manner. The humanized version of the antibody was able to inhibit VEGF production by normal human lung fibroblasts (NHLF) stimulated with 10 μg/mL recombinant CD147 for 48 hours. The humanized version of the antibody inhibited more than 40 % of VEGF production in co-culture of tumor cells and fibroblast cells. The humanized version of the antibody inhibited angiogenesis in vivo. SCID Beige Mice implanted with MDA-MB-231 human breast carcinoma cells were treated with E+5F6(MuIgG2a), the antibody inhibited tumor angiogenesis in Panc-1 matrigel plug model. The antibody showed potential as therapeutic and prophylactic agents (US8618264B2). The structure of the Fab fragment was determined (Teplyakov et al., 2010).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Andere Bezeichnung: CD147

Hintergrund: Basigin, Emmprin, 5F7, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, Hepatoma-associated antigen, HAb18G, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, TCSF,BSG, Cluster of differentiation 147

UniProt: P35613

Pathways: S100 Proteine