Rekombinanter F2RL1 Antikörper (Cleavage Site)

Produktnummer ABIN7543274

Dieses Kaninchen Chimeric-Antikörper erkennt spezifisch F2RL1 in ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter F2RL1 Antikörper (Cleavage Site) (ABIN7543274)

Target: F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser F2RL1 Antikörper ist unkonjugiert

Applikation: ELISA

Klon: Ab4999

Produktdetails anti-F2RL1 Antikörper

Bindungsspezifität: Cleavage Site

Verwendungszweck: Anti-PAR2 [Ab4999 (Par-B)], Rabbit IgG, lambda

Spezifität: This antibody has binding specificity for N-terminal PAR-2 peptide 'SKGRSLIGKVDGTSHVTGKGV' and can bind human, mouse, rat and cynomolgus monkey PAR-2 peptide. PAR2 acts as a receptor for trypsin and trypsin-like enzymes coupled to G proteins.

Kreuzreaktivität: Cynomolgus, Maus, Ratte

Produktmerkmale:

Original Species of Ab: Human

Original Format of Ab: IgG4

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated using phage display techniques to pan for antibodies (Fab fragments) capable of immunospecifically binding to residues spanning the protease cleavage site at the N-terminal of PAR-2. The affinity maturation of the selected parental clone Ab3777 resulted in generation of the antibody.

Isotyp: IgG lambda

Anwendungsinformationen

Applikationshinweise: This antibody was generated by the affinity maturation of a parental clone Ab3777. This antibody exhibited concentration-dependent binding to human PAR-2 transfected HEK293 cells with EC50 values of 0.2 nM. ELISA experiments demonstrated specific binding of this antibody to PAR-2 peptide (coupled to Transferrin as carrier). This antibody did not show any binding affinity for PAR-4 peptide or PAR-1 peptide. This antibody showed potent inhibition of calcium release in the human lung epithelial cell line A549 stimulated with trypsin similar to the parental clone Ab3777. (US20110311553).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu F2RL1

Target: F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))

Andere Bezeichnung: PAR2

Hintergrund: F2RL1, PAR-2, GPR11, Proteinase-activated receptor 2, Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like 1, Ab3777

UniProt: P55085

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interaktom