Rekombinanter TREM1 Antikörper

Produktnummer ABIN7543260

Der Kaninchen Chimeric Anti-TREM1-Antikörper wurde für FACS, ELISA und BR validiert. Er ist geeignet, TREM1 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter TREM1 Antikörper (ABIN7543260)

Target: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser TREM1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

Klon: 14F69

Produktdetails anti-TREM1 Antikörper

Verwendungszweck: Anti-TREM-1 [14F69], Rabbit IgG, kappa

Spezifität: This antibody binds human, cynomolgus monkey and rhesus monkey TREM-1. TREM1 is a receptor that is expressed on monocytes, macrophages, neutrophils and dendritic cells. It plays important roles in innate and adaptive immunity by amplifying inflammatory responses. When activated, TREM-1 associates with the ITAM-containing signaling adaptor protein, DAP12. Downstream signalling may include activation of the NFAT transcription factor, causing an upregulation of pro-inflammatory cytokine production.

Kreuzreaktivität: Cynomolgus, Rhesusaffen

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing both wild type BALB/c mice and TREM-1 knock-out (KO) mice (C57BL/6 background) with either human TREM-1, cells expressing hTREM-1 (BWZ.36 cells), or a combination of both.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The original IgG2a antibody binds human TREM-1 with an affinity of 2 X 10^-10 nM as tested by suface plasmon resonance. The screening of original antibody for binding human TREM-1 was done using ELISA and flow cytometry. This antibody is capable of binding both human, cynomolgus monkey and rhesus monkey PBMCs. This antibody was reported to block the release of TNFalpha from stimulated peripheral blood mononuclear cells (PBMCs). This antibody was also able to block the ligand-induced TREM-1 activation in such BWZ/hTREM-1 reporter cell assay (US9000127).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu TREM1

Target: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Andere Bezeichnung: TREM-1

Hintergrund: TREM1, CD354, Triggering receptor expressed on myeloid cells 1, Triggering receptor expressed on monocytes 1

UniProt: Q9NP99