Rekombinanter Aquaporin 4 Antikörper

Produktnummer ABIN7543244

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Aquaporin 4 in IP, ELISA, FACS und InhA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter Aquaporin 4 Antikörper (ABIN7543244)

Target: Aquaporin 4 (AQP4)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Aquaporin 4 Antikörper ist unkonjugiert

Applikation: Immunoprecipitation (IP), ELISA, Flow Cytometry (FACS), Inhibition Assay (InhA)

Klon: C9401

Produktdetails anti-Aquaporin 4 Antikörper

Verwendungszweck: Anti-Aquaporin 4 [C9401], Mouse IgG2b, kappa

Spezifität: This antibody recognizes and binds extracellular domains of both the M1 and M23 isoforms of human aquaporin 4 (AQP4), but not mouse aquaporin 4. AQP4 is a water channel expressed on astrocytes throughout the CNS and in skeletal muscle and some epithelial cells outside of the CNS. It plays an important role in brain water homeostasis and in glymphatic solute transport. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system (CNS) that is associated with demyelination and neurological deficits in optic nerve, spinal cord and brainstem. Most NMOSD patients are seropositive for IgG autoantibodies (NMO-IgG) directed against extracellular epitopes of aquaporin-4 (AQP4). Prevention of the binding of NMO-IgG, therefore, may alleviate the disease.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing an aquaporin 4 knock out mice with human AQP4 as an immunogen expressed on the surface of baculovirus.

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: This antibody binds extracellular domain of human aquaporin 4 with a very high binding affinity. The binding of this antibody inhibits the binding of NMO-IgG to AQP4 (PMID: 23746426). The binding affinity of this antibody for human AQP4 M23 isoform expressed on the surface of baculovirus and CHO cells was tested with ELISA (PMID: 26398585, WO2014196658). The binding characterization of this antibody was done using flow cytometry. This antibody can be used for the immunoprecipitation of human AQP4 but not mouse AQP4 (WO2014196658, PMID: 23909421). This antibody is not suitable for detection of denatured human AQP4 in a western blot assay (WO2014196658).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Aquaporin 4

Target: Aquaporin 4 (AQP4)

Andere Bezeichnung: Aquaporin 4

Hintergrund: AQP4, AQP-4, Aquaporin-4, MIWC, WCH4, Mercurial-insensitive water channel

UniProt: P55087

Pathways: Sensory Perception of Sound