Rekombinanter FOLR1 (Farletuzumab Biosimilar) Antikörper

Produktnummer ABIN7543242

This anti-FOLR1 (Farletuzumab Biosimilar) antibody (ABIN7543242) is a Rabbit Chimeric antibody detecting FOLR1 (Farletuzumab Biosimilar) in ELISA, Func, Anta, IHC, in vivo, Inh, Neut. Suitable for Human.

Kurzübersicht für Rekombinanter FOLR1 (Farletuzumab Biosimilar) Antikörper (ABIN7543242)

Target: FOLR1 (Farletuzumab Biosimilar)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser FOLR1 (Farletuzumab Biosimilar) Antikörper ist unkonjugiert

Applikation: ELISA, Functional Studies (Func), Antagonist (Anta), Immunohistochemistry (IHC), In vivo Studies (in vivo), Inhibition (Inh), Neutralization (Neut)

Produktdetails anti-FOLR1 (Farletuzumab Biosimilar) Antikörper

Verwendungszweck: Anti-FOLR1 [Farletuzumab (MORAb-003, M3)], Rabbit IgG, kappa

Spezifität: This antibody binds human folate receptor alpha (FOLR1). FOLR1 binds folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells by receptor mediated endocytosis. It is required for normal embryonic development and normal cell proliferation. The human folate receptor-alpha (FOLR1) is overexpressed in ovarian cancer but largely absent in normal tissue. It is hypothesized that the presence of elevated levels of FRα can be correlated with the propagation rate and phenotype of the tumors.

Kreuzreaktivität: Cynomolgus

Produktmerkmale:

Original Species of Ab: Human

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original parental mouse antibody was generated by immunizing (BALB/c X C57BL/6) F1 mice by intraperitoneal injection containing cultured LU-75(c) choriocarcinoma cells to produce hybridomas that generated the antibody LK26. Later on the Farletuzumab was generated by CDR grafting technique by taking CDRs of mouse parental clone LK26 and grafting them on human framework regions.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody was derived from the optimization of mouse parental LK26 antibody using a whole-cell genetic evolution platform. This antibody possesses growth-inhibitory activity on cells overexpressing FR-alpha. It elicited robust antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) in vitro and inhibited the growth of human ovarian tumor xenografts in nude mice. Immunohistochemistry studies determined that MORAb-003 infrequently stained the tubular epithelium, epithelium of the fallopian tube, and duct epithelium of the pancreas in both normal human and cynomolgus monkey tissues. Cynomolgus monkey was therefore considered an appropriate toxicology model (Ebel et al., 2007, PMID: 17346028). This antibody was also found to antagonize the activity of human FRα, resulting in the loss of growth advantage conferred by overexpression of FRα under conditions that bracket physiological (10-100 nM) folate concentrations (Routhier et al., 2006, DOI: 10.1200/jco.2006.24.18_suppl.10108). In a human ovarian cancer model, female athymic nude mice implanted with IGROV cells (expressing FRα) were treated with this antibody, Paclitaxel, or a combination of both. This antibody alone reduced tumor growth by approximately 30 % , while Paclitaxel alone reduced tumor burden by about 65 %. However, the combination of both resulted in greater than 80 % growth inhibition (Maddage et al., 2012, DOI: 10.1158/1538-7445.AM2012-4411). A phase I study in patients with platinum-resistant ovarian cancer revealed that MORAb-003 was well tolerated in patients with epithelial ovarian cancers and may have activity in platinum-resistant patients (Konner et al., 2006, DOI: 10.1200/jco.2006.24.18_suppl.5027) (Bell-McGuinn et al., 2007, DOI: 10.1200/jco.2007.25.18_suppl.5553). In a phase 2 trial in patients with platinum-sensitive ovarian cancer, patients received single-agent farletuzumab or farletuzumab combined with carboplatin and paclitaxel (or docetaxel), followed by farletuzumab maintenance until progression. Of the 47 patients who received farletuzumab, 80.9 % had normalization of their CA125, and a complete or partial objective response rate was achieved in 75 % with combination therapy (Herzog et al., 2016, DOI: 10.1200/JCO.2016.34.15_suppl.TPS5608). It was also reported that farletuzumab with carboplatin and taxane may enhance the response rate and duration of response in platinum-sensitive ovarian cancer patients with first relapse after remission of 6-18 months (Armstrong et al., 2013, PMID: 23474348).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu FOLR1 (Farletuzumab Biosimilar)

Target: FOLR1 (Farletuzumab Biosimilar)

Substanzklasse: Biosimilar

Hintergrund: FRA, Frα, Fr-α, FBP, LK26, Folate receptor 1, Folate receptor alpha, FR-alpha, Adult folate-binding protein, Ovarian tumor-associated antigen MOv18, KB cells FBP, Folate receptor adult, MOv18

UniProt: P15328