Rekombinanter Desmoglein 3 Antikörper

Produktnummer ABIN7543212

Der Kaninchen Chimeric Anti-Desmoglein 3-Antikörper wurde für ELISA, IF und IP validiert. Er ist geeignet, Desmoglein 3 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Desmoglein 3 Antikörper (ABIN7543212)

Target: Desmoglein 3 (DSG3)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser Desmoglein 3 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: AK23

Produktdetails anti-Desmoglein 3 Antikörper

Verwendungszweck: Anti-Desmoglein 3 [AK23], Rabbit IgG, kappa

Spezifität: This antibody binds mouse Dsg3 and also shows reactivity for human Dsg3. It does not show any affinity towards Dsg1. AK23 recognizes a calcium-dependent conformational epitope on Dsg3, which consisted of the V3, K7, P8, and D59 Dsg3-specific residues that formed the adhesive interface between juxtaposed Dsg.

Kreuzreaktivität: Maus

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing Dsg3 knockout mice with recombinant mouse Dsg3-His in complete Freund's adjuvant subcutaneously.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody can be used to study the blister formation and complex interaction that occur in autoimmune blistering disease like Pemphigus vulgaris (PV). Pemphigus vulgaris (PV) is an epidermal blistering disorder caused by antibodies directed against the desmosomal cadherin desmoglein-3 (Dsg3). AK23 was reported to induce pemphigus vulgaris (PV) blisters in neonatal mice. The binding affinity of this antibody for human and mouse desmoglein 3 was determined using ELISA. This antibody was also used in the live keratinocyte staining of mouse PAM212 cell line and indirect immunofluorescent staining of mouse oral mucuous membrance, mouse liver and lower parts of the human epidermis. Immunoprecipitaton was used for the determination of the epitope of the antibody (PMID: 12574390). This antibody was also used in the study of alteration in desmosome morphology (PMID: 23226536).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Desmoglein 3

Target: Desmoglein 3 (DSG3)

Andere Bezeichnung: Desmoglein 3

Hintergrund: DSG3, PVA, CDHF6, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6

UniProt: P32926