Rekombinanter TPBG Antikörper

Produktnummer ABIN7543174

Dieses Anti-TPBG-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TPBG in ELISA, IHC, IP, IF, DB und RIA. Geeignet für Human.

Kurzübersicht für Rekombinanter TPBG Antikörper (ABIN7543174)

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TPBG Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB), Radioimmunoassay (RIA)

Klon: 5T4

Produktdetails anti-TPBG Antikörper

Verwendungszweck: Anti-5T4 [5T4 (H8)], Mouse IgG1, kappa

Spezifität: This antibody binds 5T4 antigen expressed on various carcinomas. It is reported that epitope recognized by this antibody is present in the extracellular domain of the molecule more particularly towards the membrane proximal leucine rich repeat 2 (LRR2) (PMID: 11903056).

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing male BALB/c mice with wheat germ agglutinin (WGA) purified glycoproteins from deoxycholate (DOC) solubilised human syncytiotrophoblast plasma membrane (StMPM).

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: This antibody binds 5T4 antigen with a very high affinity of K(D)=1.8x10(-11) M (PMID: 11113573). The reactivity of this antibody to solubilised human syncytiotrophoblast plasma membrane (StMPM) was checked using immunodotting on nitrocellulose membrane and ELISA. The distribution of the 5T4 antigen was determined using mAb 5T4 by indirect immunoperoxidase staining of sections of normal organ and placental tissues as well as immunofluorescence and radiobinding assays with a wide variety of cell lines representing differing normal and tumour cell types. Immunoprecipitation of radiolabelled StMPM using this antibody indicated that 5T4 Molecules are glycoproteins of mol. wt of approximately 72 kD on SDS-PAGE (PMID: 3355761). This paper (PMID: 2404511) studies the immunohistochemical distribution of 5T4 antigen and reports that 54T4 antigen is present in a wide range of tumor cells including carcinomas of the bladder, breast, cervix, endometrium, lung, oesophagus, ovary, pancreas, stomach and testicular non-seminomatous germ cell tumours. 5T4 can serve as a marker for detection of tumor. Various papers report the use of this antibody for the study of expression of 5T4 antigen in various types of cancers (PMID: 2404512, 1419629, 8180020, 11578488).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu TPBG

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Andere Bezeichnung: 5T4

Hintergrund: TPBG, M6P1, WAIF1, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncofetal trophoblast glycoprotein, 5T4 oncotrophoblast glycoprotein, Wnt-activated inhibitory factor 1

UniProt: Q13641