Rekombinanter SARS-CoV-2 Nucleocapsid Antikörper

Produktnummer ABIN7543169

Dieses Kaninchen Chimeric-Antikörper erkennt spezifisch SARS-CoV-2 Nucleocapsid in ELISA, WB, FACS und IHC. Er zeigt eine Reaktivität gegenüber SARS Coronavirus-2 (SARS-CoV-2).

Kurzübersicht für Rekombinanter SARS-CoV-2 Nucleocapsid Antikörper (ABIN7543169)

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Antikörpertyp: Recombinant Antibody

Reaktivität: SARS Coronavirus-2 (SARS-CoV-2)

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser SARS-CoV-2 Nucleocapsid Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Klon: 1C7C7

Produktdetails anti-SARS-CoV-2 Nucleocapsid Antikörper

Verwendungszweck: Anti-Covid-19 & SARS-CoV Nucleocapsid [1C7C7], Rabbit IgG, kappa

Spezifität: This antibody specifically targets an epitope on the SARS-CoV-2 and SARS-CoV nucleocapsid protein and does not cross-react with the nucleocapsid of other common human coronaviruses, OC43 and 229E.

Kreuzreaktivität: SARS Coronavirus (SARS-CoV)

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: The original version of this antibody was raised against SARS-CoV-1/2.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The antibody 1C7C7 detects SARS-CoV-2 and SARS-CoV-1 and can be used in affinity binding assays, flow cytometry, immunocytochemistry, and western blotting. Its effectiveness in detecting the nucleocapsid of the novel coronavirus causing COVID-19 was confirmed in multiple studies. For instance, it was used to analyze SARS-CoV-2 nucleoprotein extracted from infected cells via Western blot (Hoagland et al., 2020) ,and similarly by Blanco-Melo et al. in a study on imbalanced host response to SARS-CoV-2 (2020, pmid: 32416070). Another group has used this clone to stain infected cells fixed with 10 % neutral formalin during a neutralization assay (Piepenbrink et al., 2020). Daniloski et al. performed similar staining procedure with 1C7C7 for a trans-complementation assay (2020, pmid: 33147445). Finally, this antibody was also used to stain SARs-CoV-2 infected cells in a study on in vitro susceptibility to SARS-CoV-2 infection (Dobrindt et al., 2020, pmid: 32995783).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Andere Bezeichnung: SARS-CoV-2 Nucleocapsid

Substanzklasse: Viral Protein

Hintergrund: Nucleoprotein, NP, NC, Protein N, Nucleocapsid protein, SARS-CoV Protein N, SARS-CoV-2 Nucleocapsid protein, SARS Coronavirus, SARS-CoV-2, SARS CoV 2, 2019-nCoV, 1C7, severe acute respiratory syndrome virus

UniProt: P0DTC9