Rekombinanter Fc gamma RII (CD32) Antikörper

Produktnummer ABIN7543154

Dieses Kaninchen Chimeric-Antikörper erkennt spezifisch in FACS, IHC, ELISA, IF und BR. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter Fc gamma RII (CD32) Antikörper (ABIN7543154)

Target: Fc gamma RII (CD32)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Blocking Reagent (BR)

Klon: MDE-9

Produktdetails

Verwendungszweck: Anti-CD32 [MDE-9], Rabbit IgG, kappa

Spezifität: This antibody recognizes and binds Fc-gamma receptor IIa (FcγRIIa) or CD32a and, more particularly, the FcγRIIa-H131 allotype.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund's adjuvant.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: MDE-9 was able to detect FcgammaRIIa-H131 in both flow cytometry and immunohistochemistry. MDE-9 bound FcgRIIa-H131 on cell lines expressing FcgRIIaH131, as well as on peripheral blood monocytic cells and PMN in immunofluorescence assays. MDE-9 was used to develop an FcgammaRIIa allotyping method based on flow cytometry. The results of flow cytometric FcgammaRIIa allotyping correlated completely with PCR genotyping. This novel allotyping assay can facilitate the screening of patients in a routine diagnostic setting. A combination of MDE-9 and mAb 41H16 can be used in FcgammaRIIa-H/H131 homozygous individuals to detect FcgammaRIIa and FcgammaRIIb surface expression on monocytes (PMID: 15604023).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Antigendetails

Target: Fc gamma RII (CD32)

Andere Bezeichnung: CD32

Hintergrund: CDw32, Fc-gamma RII-a, FcγRIIa, FcγRII, FcRII-a, FCGR2A, FcgammaRIIa-H131, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a

UniProt: P12318