Rekombinanter Fc gamma RII (CD32) Antikörper

Produktnummer ABIN7543151

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in FACS, ELISA und BR. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter Fc gamma RII (CD32) Antikörper (ABIN7543151)

Target: Fc gamma RII (CD32)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

Klon: MDE-8

Produktdetails

Verwendungszweck: Anti-CD32 [MDE-8], Mouse IgG1, kappa

Spezifität: This antibody recognizes and binds Fc-gamma receptor IIa (FcγRIIa) or CD32a on peripheral blood monocytes, polymorphonuclear cells and platelets.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund's adjuvant.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The binding ability of MDE-8 to FcgammaRIIa was confirmed using flow cytometry. MDE-8 binds to FcgammaRIIa on cell lines, peripheral bloodmonocytes, PMN, platelets and blocked ligand binding via this receptor. It can also effectively blocked FcγRIIa ligand-binding via its F(ab')2 fragment. Overnight incubation of monocytes with F(ab')2 fragments of MDE-8 leads to a 60 % decrease in cell surface expression of FcγRIIa. MDE-8 whole antibody induced a concomitant 30 % decrease of FcγRI on THP-1cells and monocytes (PMID: 15982355). MDE-8 was reoported to induce Fc-mediated hypothermia and severe thrombocytopenia in FCGR2A mice (PMID: 26396093).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Antigendetails

Target: Fc gamma RII (CD32)

Andere Bezeichnung: CD32

Hintergrund: CDw32, Fc-gamma RII-a, FcγRIIa, FcγRII, FcRII-a, FCGR2A, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a

UniProt: P12318