Rekombinanter T Cell Receptor Antikörper
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- Target
- T Cell Receptor
- Antikörpertyp
- Recombinant Antibody
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Chimeric
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Konjugat
- Unkonjugiert
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Applikation
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Blocking Reagent (BR)
- Verwendungszweck
- Anti-TCR [BMA031], Rabbit IgG, kappa
- Spezifität
- This antibody reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. TCR-αβ is expressed by the majority of mature CD3+ T cells.
- Produktmerkmale
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Original Species of Ab: Mouse
Original Format of Ab: IgG2b
- Aufreinigung
- Protein A affinity purified
- Immunogen
- The original antibody was generated by immunizing mice with human perihperal blood T-lymphocytes.
- Klon
- BMA031
- Isotyp
- IgG kappa
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- Applikationshinweise
- This antibody is recomended for detection of viable human αβ+ TCR lymphocyte. It is highly effective in modulating immune reactions and is capable of activating human T lymphocytes in vito (PMID: 3092794). Double immunofluorescence staining was used to ascertain that this antibody does not cross react with γδ+ TCR cells. FACS analysis confirmed that BMA031 did not react with γδ+ TCR cells. BMA031 successfully precipitates TCR αβ/CDR complex (PMID: 1704361). BMA031 in conjugation with triple drug induction treatment in kidney-transplanted patients showed no side effects and reduction in graft rejections (PMID: 2978369). A clinical phase II trial administered 50 mg of murine anti-human alpha/beta-TCR mAb (BMA 031) intravenously on the day of, as well as 2 and 4 days after, cadaveric donor renal transplantation in combination with a CsA/prednisone regimen. After a minimum of 30 months follow-up, the actual allograft survival rate was 87 % in the BMA-treated group compared with 68 % in the control cohort (PMID: 8009591).Humanized version of this antibody BMA 031-EUCIV3 is found to have 2.5 times less affinity than its murine parent, however the ability to cause antibody dependent cell-mediated cytolysis was significantly enhanced (PMID: 1836485).
- Kommentare
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Target
- T Cell Receptor
- Andere Bezeichnung
- TCR
- Hintergrund
- Human α/β T Cell Receptor (TCR)
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