Rekombinanter CD68 Antikörper

Produktnummer ABIN7543090

Dieses Anti-CD68-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CD68 in FACS, IHC und IP. Geeignet für Maus.

Kurzübersicht für Rekombinanter CD68 Antikörper (ABIN7543090)

Target: CD68 (CD68 Molecule (CD68))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD68 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Klon: FA-11

Produktdetails anti-CD68 Antikörper

Verwendungszweck: Anti-CD68 [FA-11], Rabbit IgG, kappa

Spezifität: This antibody is specific for murine CD68, known also as macrosialin (MS). CD68 is a 110 kDa, heavily O- and N- glycosylated type I integral membrane protein belonging to the sialomucin family and is closely related to the lysosomal/endosomal-associated membrane glycoproteins (LAMP). CD68 is predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several acute myeloid leukemia studies.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising rats (AO x Lou strain) with murine macrosialin (MS).

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: When it was first generated, this antibody was used in binding assays, immunoprecipitation (IP) and immunofluorescence (IF) to study the differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes (Smith et al., 1987). Subsequently, this antibody has been utilised in numerous FACS, IP, IHC, ICC/IF, WB applications, for instance, to delineate the roles of mouse and human CD68 as macrophage receptors for oxidised low density lipoprotein (Ramprasad et al., 1996), to indicate that mice unresponsive to granulocyte-macrophage colony-stimulating factor (GM-CSF) are unexpectedly resistant to cutaneous Leishmania major infection (Scott et al., 2000), to characterise the macrophage subsets harbouring Leishmania donovani in spleens of infected BALB/c mice (Lang et al., 2000), and to examine the role of Foxp3(+) Tregs in Alzheimer's disease (Baruch et al., 2015).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD68

Target: CD68 (CD68 Molecule (CD68))

Andere Bezeichnung: CD68

Hintergrund: Macrosialin, FA/11, FA11 antigen

UniProt: P31996