Rekombinanter CD4 Antikörper

Produktnummer ABIN7543051

Der Kaninchen Chimeric anti-CD4 Antikörper (Klon OX-68) (ABIN7543051) detektiert spezifisch CD4 in FACS, WB, IP, SPR und Bind. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für Rekombinanter CD4 Antikörper (ABIN7543051)

Target: CD4

Antikörpertyp: Recombinant Antibody

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Surface Plasmon Resonance (SPR), Binding Studies (Bind)

Klon: OX-68

Produktdetails anti-CD4 Antikörper

Verwendungszweck: Anti-CD4 [OX-68], Rabbit IgG, kappa

Spezifität:

This antibody is reactive with rat CD4 domain 3 & 4, and is partly competitive with OX-67 & 69.

Application Notes original Clone: This antibody binds to rat CD4. Upon challenge with HIV-1, this antibody was able to significantly inhibit viral entry into HeLa cells expressing a mutant of rat CD4 that binds gp120 (SImon, 1997). Similarly, pre-binding of gp120 resulted in a 50 % reduction in binding of this antibody, indicating epitope masking. Analogous effects on viral entry and synctium formation are observed with MAbs against human CD4.

Produktmerkmale: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was prepared by subcutaneously injecting BALB/c mice with a soluble form of rat CD4, consisting of domains 3 and 4. A boost of a mixture of free sCD4 and sCD4 bound to cells was administered intravenously.

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4

Hintergrund: Leu3/T4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, W3/25 antigen

UniProt: P05540

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events