Rekombinanter CD43 Antikörper

Produktnummer ABIN7543048

Dieses Anti-CD43-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CD43 in FACS, WB und RIA. Geeignet für Ratte.

Kurzübersicht für Rekombinanter CD43 Antikörper (ABIN7543048)

Target: CD43 (SPN) (Sialophorin (SPN))

Antikörpertyp: Recombinant Antibody

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD43 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Radioimmunoassay (RIA)

Klon: OX-58

Produktdetails anti-CD43 Antikörper

Verwendungszweck: Anti-CD43 [OX-58], Rabbit IgG, kappa

Spezifität: This antibody recognises rat leukosialin, a major cell-surface sialoglycoprotein of thymocytes and T lymphocytes. It is also expressed on neutrophils, bone marrow stem cells and B-lineage cells after activation. The specificity of the leukosialin epitope is largely determined by linear protein sequence, as indicated by the reaction of this antibody with the extracellular domain of leukosialian expressed in an unglycosylated form in E. coli. However, the sensitivity of this antibody to neuraminidase treatment of leukosialin indicates that glycosylation can modify epitope conformation or flexibility.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was prepared by immunizing BALB/c mice with rat thymocyte leukosialin and thymocytes.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody binds to rat leukosialin, and can be used to label thymocytes and T lymphocytes in the lymph node cell population in flow cytometry analysis. This antibody has also been used, along with other anti-CD43 MABs, in a Western blot analysis to localize leukosialin epitopes.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Andere Bezeichnung: CD43

Hintergrund: Leukosialin, Leukocyte sialoglycoprotein, Sialophorin, W3/13 antigen, OX 58, MRC OX 58

UniProt: P13838

Pathways: Regulation of Leukocyte Mediated Immunity