Rekombinanter CD200R1 Antikörper

Produktnummer ABIN7543015

Dieses Anti-CD200R1-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CD200R1 in WB, FACS, IP, IHC (fro) und BR. Geeignet für Ratte.

Kurzübersicht für Rekombinanter CD200R1 Antikörper (ABIN7543015)

Target: CD200R1 (CD200 Receptor 1 (CD200R1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD200R1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Klon: OX-102

Produktdetails anti-CD200R1 Antikörper

Verwendungszweck: Anti-CD200R [OX-102], Rabbit IgG, kappa

Spezifität: This antibody is specific for CD200R (OX2R), a cell-surface receptor for OX2 restricted to cells of the myeloid lineage, macrophages isolated from the peritoneal cavity, lung, spleen, and leukocyte dendritic cells are highly positive in OX102 mAb staining tests.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: 6 week-old male BALB/c mice immunized six times with either 0.1-0.25 mg of a crude Tween-40 membrane fraction solubilized from rat (PVG) thioglycollate-elicited peritoneal cells or unlysed whole cells.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody binds the rat OX2 receptor, and thus can be used to stain rat resident peritoneal cells (RPCs), as well as macrophages from the lung, spleen and brain, and leukocyte dendritic cells (Wright, 2000). In addition, this antibody can be used to block the binding of OX2-coated beads to rOX2R on rat macrophages and to human kidney endothelial cells transfected with rOX2R cDNA. This antibody can also be used to purify the rOX2R antigen by immunoprecipitation from rat splenic lysates. Finally, the use of this antibody to block the OX2-OX2R interaction in rats with experimental allergic encephalomyelitis exacerbates the disease, and indicates a role for the OX2-OX2R interaction in the regulation of macrophage/microglia activity in vivo.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD200R1

Target: CD200R1 (CD200 Receptor 1 (CD200R1))

Andere Bezeichnung: CD200R

Hintergrund: OX2R, CD200, OX2

UniProt: Q9ES58