Rekombinanter CA9 Antikörper

Produktnummer ABIN7542959

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CA9 in WB, IHC, ELISA, IF, FACS, BP und Crys. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter CA9 Antikörper (ABIN7542959)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CA9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Blocking Peptide (BP), Crystallization (Crys)

Klon: M75

Produktdetails anti-CA9 Antikörper

Verwendungszweck: Anti-Carbonic Anhydrase IX [M75], Mouse IgG1, kappa

Spezifität: This antibody is specific for the sequence PGEEDLP, which is repeated several times between the 61st and 96th aa residues of human carbonic anhydrase IX's PG domain.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/C mice with MaTu-infected HeLa cells.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The original format of this antibody (mouse IgG2b) was characterized by and used in IB (WB), IF, and FC assays (Pastoreková et al., 1992, PMID: 1312272). This antibody's epitope has been determined by a competitive ELISA against the peptide APGEEDLPA. Furthermore, it was used for WB and was shown to blocks the capacity of immobilized MN/CA IX to attach cells (Závada et al., 2000, PMID: 10839295). The Fab version of this antibody was crystallized and analyzed. Additionally, an scFv version of this antibody was created, and its thermodynamic parameters against the peptide DLPGEEDLPGEE were evaluated via ITC. Its K_d was determined to be 0.42 μM (Král et al., 2008, PMID: 18041760). This antibody was used for WB in breast cell lines and for IHC in xenograft tumors grown from PC-3 cells in mice (Li et al., 2009, PMID: 19538935).

Kommentare:

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Andere Bezeichnung: Carbonic Anhydrase IX

Hintergrund: CA9, CAIX, MN/CA IX, endogenous protein p54/58N

UniProt: Q16790