Tyrosine Hydroxylase Antikörper (pSer40)

Produktnummer ABIN7540679

Hersteller Produkt- Nr.: 600-401-c99

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Tyrosine Hydroxylase in WB, IHC, ELISA und FM. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für Tyrosine Hydroxylase Antikörper (pSer40) (ABIN7540679)

Target: Tyrosine Hydroxylase (TH)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tyrosine Hydroxylase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Produktdetails anti-Tyrosine Hydroxylase Antikörper

Bindungsspezifität: pSer40

Hersteller Produkt- Nr.: 600-401-c99

Hersteller: Rockland

Verwendungszweck: Tyrosine Hydroxylase phospho S40 Antibody

Kreuzreaktivität (Details): Expect reactivity with the following species based on sequence homology: mouse.

Aufreinigung: Anti-Tyrosine Hydroxylase pS40 antibody is affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-Tyrosine Hydroxylase pS40 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser 40 conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:1000

IF_Microscopy_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Tyrosine Hydroxylase pS40 antibody is tested for use in ELISA, Western Blotting, and ICC/IHC and suitable for IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 42/44 kDa in size corresponding to the tyrosine hydroxylase proteins phosphorylated at Ser40 in the appropriate cell lysate or extract. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Andere Bezeichnung: Tyrosine Hydroxylase

Hintergrund: Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH,Tyrosine Hydroxylase pS40 antibody recognizes Tyrosine hydroxylase (TH) which is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse. TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling. The activity of TH is also regulated by phosphorylation. Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs. Tyrosine Hydroxylase pS40 antibody is ideal for investigators involved in Neuronal Circuitry and disorders, and more generally in Neuroscience.

Gen-ID: 25085

NCBI Accession: NP_036872

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour