SARS-CoV-2 Spike Antikörper (RBD)

Produktnummer ABIN7540678

Hersteller Produkt- Nr.: 600-401-ms8s

Dieses Anti-SARS-CoV-2 Spike-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SARS-CoV-2 Spike in ELISA, IF und FM. Geeignet für SARS Coronavirus-2 (SARS-CoV-2). Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für SARS-CoV-2 Spike Antikörper (RBD) (ABIN7540678)

Target: SARS-CoV-2 Spike

Reaktivität: SARS Coronavirus-2 (SARS-CoV-2)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SARS-CoV-2 Spike Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-SARS-CoV-2 Spike Antikörper

Bindungsspezifität: RBD

Hersteller Produkt- Nr.: 600-401-ms8s

Hersteller: Rockland

Verwendungszweck: SARS-CoV-2 Spike Protein (RBD) Antibody

Kreuzreaktivität (Details): Anti-SARS-CoV-2 Spike affinity purified antibody is directed against SARS Coronavirus 2 Spike protein.

Aufreinigung: The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 Spike resin.

Sterilität: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the SARS Coronavirus Spike Protein within the Receptor Binding Domain (RBD).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:150,000 - 1:450,000

IF_Microscopy_Dilution: 1:1000

Kommentare:

Suggested Applications: Other
Anti-SARS-CoV-2 Spike protein purified antibody has been tested for use in ELISA and IF. This antibody shows reactivity to recombinant SARS COV-2, S1 subunit protein and recombinant SARS COV-2 whole spike protein.  Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-SARS-CoV-2 Spike Antikörper (ABIN7540678)

Kaleta, Kern, Hong, Hölzer, Kochs, Beer, Schnepf, Schwemmle, Bollen, Kolb, Huber, Ulferts, Weigang, Dudas, Wittig, Jaki, Padane, Lagare, Salou, Ozer, Nnaemeka, Odoom, Rutayisire, Benkahla, Akoua-Koffi, Ouedraogo, Simon-Lorière, Enouf, Kröger, Calvignac-Sp: "Antibody escape and global spread of SARS-CoV-2 lineage A.27." in: Nature communications, Vol. 13, Issue 1, pp. 1152, (2022) (PubMed).

Weigang, Fuchs, Zimmer, Schnepf, Kern, Beer, Luxenburger, Ankerhold, Falcone, Kemming, Hofmann, Thimme, Neumann-Haefelin, Ulferts, Grosse, Hornuss, Tanriver, Rieg, Wagner, Huzly, Schwemmle, Panning, Kochs: "Within-host evolution of SARS-CoV-2 in an immunosuppressed COVID-19 patient as a source of immune escape variants." in: Nature communications, Vol. 12, Issue 1, pp. 6405, (2021) (PubMed).

Wang, Wang, Gupta, Morrissey, Naik, Singamaneni: "Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation." in: Advanced healthcare materials, Vol. 10, Issue 18, pp. e2100410, (2021) (PubMed).

Details zu SARS-CoV-2 Spike

Target: SARS-CoV-2 Spike

Andere Bezeichnung: SARS-CoV-2 Spike Protein

Hintergrund: Rabbit anti-SARS CoV 2 Spike Protein Receptor Binding Domain Antibody, Spike glycoprotein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, S glycoprotein, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2),SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein, and other non-structural proteins (NSPs). Spike proteins located on the surface of the virus, have a high affinity for binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial which serves as an entry mechanism for infection. As such, the S protein may be vital to blocking viral infection and vaccine development. Anti-SARS-CoV-2 Spike Protein Antibody is useful for researchers interested in diagnostics, vaccines, and viral research.

UniProt: P0DTC2