SARS-CoV-2 NSP8 Antikörper

Produktnummer ABIN7540672

Hersteller Produkt- Nr.: 100-401-a53

Dieses Anti-SARS-CoV-2 NSP8-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SARS-CoV-2 NSP8 in WB, IF, FM und IP. Geeignet für SARS Coronavirus (SARS-CoV). Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für SARS-CoV-2 NSP8 Antikörper (ABIN7540672)

Target: SARS-CoV-2 NSP8 (NSP8) (SARS-CoV-2 Non-Structural Protein 8 (NSP8))

Reaktivität: SARS Coronavirus (SARS-CoV)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SARS-CoV-2 NSP8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM), Immunoprecipitation (IP)

Produktdetails anti-SARS-CoV-2 NSP8 Antikörper

Hersteller Produkt- Nr.: 100-401-a53

Hersteller: Rockland

Verwendungszweck: Sars-Cov Nonstructural Protein 8 Antibody

Kreuzreaktivität (Details): This antibody is directed against SARS-Coronavirus nsp8 protein.

Aufreinigung: The product is neat antiserum.

Sterilität: Sterile filtered

Immunogen: This whole rabbit serum was produced by repeated immunizations with a purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: 1:60

IF_Microscopy_Dilution: 1:300

Western_Blot_Dilution: 1:1,000

Other: IMMUNOELECTRON MICROSCOPY 1:100

Kommentare:

This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C.  Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mM HEPES/2% FCS. Cells were fixed with PBS/3% PFA.  After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-SARS-CoV-2 NSP8 Antikörper (ABIN7540672)

Shi, Yu, Li, Cui, Zhao, Wang, Wang, Zhang, Huang: "Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells." in: Microbiology spectrum, Vol. 10, Issue 4, pp. e0074422, (2022) (PubMed).

Details zu SARS-CoV-2 NSP8

Target: SARS-CoV-2 NSP8 (NSP8) (SARS-CoV-2 Non-Structural Protein 8 (NSP8))

Andere Bezeichnung: SARS-CoV-2 NSP8

Hintergrund: Rabbit anti-Sars-Cov Nonstructural Protein 8 Antibody, Replicase polyprotein 1a, ORF1a polyprotein, nsp8,The nonstructural protein 8 (nsp8) is one of the SARS-Coronavirus replicase cleaving products, encoded by ORF1a. Nsp8 is thought to be part of the viral replication complex, which is associated with intracellular membranes.  No specific information on the function of nsp8 is available. Anti-SARS-CoV Nonstructural Protein 8 (nsp8) Antibody is useful for researchers interested in viral research.

Gen-ID: 1489680, 30124074

UniProt: P0C6U8