SARS-CoV-2 NSP13 Antikörper (AA 584-601)

Produktnummer ABIN7540671

Hersteller Produkt- Nr.: 100-401-a54

Der Kaninchen Polyklonal Anti-SARS-CoV-2 NSP13-Antikörper wurde für WB, FM und IP validiert. Er ist geeignet, SARS-CoV-2 NSP13 in Proben von SARS Coronavirus (SARS-CoV) zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für SARS-CoV-2 NSP13 Antikörper (AA 584-601) (ABIN7540671)

Target: SARS-CoV-2 NSP13 (HEL) (SARS-CoV-2 Helicase (NSP13) (HEL))

Reaktivität: SARS Coronavirus (SARS-CoV)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SARS-CoV-2 NSP13 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Fluorescence Microscopy (FM), Immunoprecipitation (IP)

Produktdetails anti-SARS-CoV-2 NSP13 Antikörper

Bindungsspezifität: AA 584-601

Hersteller Produkt- Nr.: 100-401-a54

Hersteller: Rockland

Verwendungszweck: Sars-Cov Nonstructural Protein 13 Antibody

Kreuzreaktivität (Details): This antibody is directed against SARS-Coronavirus nsp13 protein.

Aufreinigung: The product is neat antiserum.

Sterilität: Sterile filtered

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a BSA-coupled synthetic peptide corresponding to the C-terminus (amino acid residues 584-601) of the SARS Coronavirus nonstructural protein 13.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: 1:60

IF_Microscopy_Dilution: 1:800

Western_Blot_Dilution: 1:1,000

Other: IMMUNOELECTRON MICROSCOPY 1:300

Kommentare:

Suggested Applications: IF, Multiplex
This antibody is suitable for use in western blotting, immunofluorescence microscopy and immunoelectron microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 67 kDa in size corresponding to SARS-CoV nsp13 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C.  Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mMHEPES/2% FCS. Cells were fixed with PBS/3% PFA.  After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-SARS-CoV-2 NSP13 Antikörper (ABIN7540671)

Shi, Yu, Li, Cui, Zhao, Wang, Wang, Zhang, Huang: "Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells." in: Microbiology spectrum, Vol. 10, Issue 4, pp. e0074422, (2022) (PubMed).

Ivanov, Thiel, Dobbe, van der Meer, Snijder, Ziebuhr: "Multiple enzymatic activities associated with severe acute respiratory syndrome coronavirus helicase." in: Journal of virology, Vol. 78, Issue 11, pp. 5619-32, (2004) (PubMed).

Details zu SARS-CoV-2 NSP13

Target: SARS-CoV-2 NSP13 (HEL) (SARS-CoV-2 Helicase (NSP13) (HEL))

Andere Bezeichnung: SARS-CoV-2 NSP13

Hintergrund: Rabbit anti-Sars-Cov Nonstructural Protein 13 Antibody, Replicase polyprotein 1a, pp1a antibody, Growth factor-like peptide,The coronavirus nonstructural protein 13 (nsp13) is one of the SARS-Coronavirus replicase cleaving products encoded by ORF1b. Nsp13 is thought to be part of the viral replication complex, which is associated with intracellular membranes. Nsp13 contains a C-terminal NTPase/Helicase domain and an N-terminal putative zinc-binding motif. Anti-SARS-CoV Nonstructural Protein 13 (nsp13) Antibody is useful for researchers interested in viral research.

Gen-ID: 1489680, 30124074

UniProt: P0C6U8