T7 RNA Polymerase Antikörper

Produktnummer ABIN7540668

Hersteller Produkt- Nr.: 200-401-mx5

Der Kaninchen Polyklonal anti-T7 RNA Polymerase Antikörper wird verwendet zum Nachweis von T7 RNA Polymerase in Proben von Bacteriophage T7. Er wurde validiert für ELISA und WB.

Kurzübersicht für T7 RNA Polymerase Antikörper (ABIN7540668)

Target: T7 RNA Polymerase

Reaktivität: Bacteriophage T7

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB)

Produktdetails

Hersteller Produkt- Nr.: 200-401-mx5

Hersteller: Rockland

Verwendungszweck: T7 RNA Polymerase Antibody

Kreuzreaktivität (Details): Protein A purified T7 RNA Polymerase antibody is directed against T7 Polymerase (E.coli)

Sterilität: Sterile filtered

Immunogen: Anti-T7 Polymerase antibody was produced from whole rabbit serum produced by repeated immunizations with T7 Polymerase isolated from E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:11,000-1:13,000

Western_Blot_Dilution: 1.0 μg/mL

Kommentare:

This protein A purified Anti-T7 RNA Polymerase antibody has been tested for use in ELISA and Western Blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 98.9 kDa in size corresponding to T7 Polymerase by western blotting in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: T7 RNA Polymerase

Hintergrund: Escherichia phage T7 (Bacteriophage T7), DNA-directed RNA polymerase, DNA-dependent RNA polymerase, RNA nucleotidyltransferase (DNA-directed),T7 RNA Polymerase is a highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. It recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. T7 RNA Polymerase unwinds the double-stranded DNA to expose the coding strand for templating. It participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Also, T7 RNA Polymerase plays a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process. In eukaryotes three forms of this enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized.

Gen-ID: 1261050

NCBI Accession: NP_041960

UniProt: P00573