T4 RNA Ligase Antikörper

Produktnummer ABIN7540667

Hersteller Produkt- Nr.: 200-401-mx4

Produktdetails

Target: T4 RNA Ligase

Reaktivität: Bacteriophage T4

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA

Hersteller Produkt- Nr.: 200-401-mx4

Hersteller: Rockland

Verwendungszweck: T4 RNA Ligase Antibody

Kreuzreaktivität (Details): Protein A purified T4 RNA Ligase antibody is directed against T4 RNA Ligase (E.coli)

Sterilität: Sterile filtered

Immunogen: Anti-T4 RNA Ligase antibody was produced from whole rabbit serum produced by repeated immunizations with T4 Ligase isolated from E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optional[Neutralization_Dilution]: 1:13,000-1:14,000

Kommentare:

This protein A purified Anti-T4 RNA Ligase antibody has been tested for use in ELISA.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43.5 kDa in size corresponding to RNA Ligase by western blotting in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: T4 RNA Ligase

Hintergrund: Enterobacteria phage T4, RNA ligase 1, polyribonucleotide synthase (ATP), ribonucleic ligase,The T4 RNA Ligase enzyme catalyzes the ligation of RNA strands with 3'-hydroxyl and 5'-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in RNA. This catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a lysine residue. The adenylate group is then transferred to the 5'-phosphate terminus of the substrate, forming the capped structure 5'-(5'-diphosphoadenosine)-[RNA]. Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH terminus on the capped terminus, which results in the formation of the phosphodiester bond and release of the adenylate.

Gen-ID: 258717

NCBI Accession: NP_049839

UniProt: A0A7S9SW04