Nuclease Antikörper

Produktnummer ABIN7540666

Hersteller Produkt- Nr.: 200-401-mu6

Dieses Anti-Nuclease-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Nuclease in ELISA und WB. Geeignet für Serratia marcescens.

Kurzübersicht für Nuclease Antikörper (ABIN7540666)

Target: Nuclease

Reaktivität: Serratia marcescens

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nuclease Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Produktdetails anti-Nuclease Antikörper

Hersteller Produkt- Nr.: 200-401-mu6

Hersteller: Rockland

Verwendungszweck: Nuclease/NucA Antibody

Kreuzreaktivität (Details): This antibody will react with endonuclease from S.

Aufreinigung: This antibody was purified from monospecific antiserum by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen: Anti-Nuclease/NucA was prepared by repeated immunizations with recombinant benzonase protein produced in E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:21,600 - 1:22,600

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Nuclease/NucA has been tested by ELISA and western blot. Expect a band ~ 28 kDa in size corresponding to endonuclease by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Nuclease

Target: Nuclease

Andere Bezeichnung: nucA

Hintergrund: Benzonase, Nuclease, Endonuclease, Serratia marcescens endonuclease, NucA,Nucleases are secreted by Serratia marcescens into the medium it surrounds. The enzyme is a sugar-nonspecific hydrolase, capable of cleaving both RNA and DNA in either double or single stranded form. It requires divalent cations, preferably Mg2+, and is functional across a broad pH range from 6 to 10 (optimal at 8-8.5) and wide temperature ranges between 35 °C and 44 °C. The ability of Serratia to secrete nuclease appears to be regulated. Bacterial cultures at differing cell densities display different kinetics and efficiencies of nuclease secretion [i.e. growth medium, growth conditions, and host cell mutations]. Anti-Nuclease/NucA Antibody is useful for researcher interested in identifying nucleic acid contamination.

UniProt: P13717