SNAIL Antikörper (Internal Region)

Produktnummer ABIN7540622

Hersteller Produkt- Nr.: 600-401-mm5s

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SNAIL in WB, ELISA, IHC, IF und FM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SNAIL Antikörper (Internal Region) (ABIN7540622)

Target: SNAIL (SNAI1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SNAIL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-SNAIL Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-mm5s

Hersteller: Rockland

Verwendungszweck: SNAI1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human SNAI1.

Aufreinigung: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-SNAI1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human SNAI1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:300

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:2000

Kommentare:

Suggested Applications: User Optimized
Anti-SNAI1 Antibody has been tested in ELISA, WB, IHC, and IF. Expect a band at ~29 in western blot using appropriate lysates. Positive control used: SNAI1 overexpressed HEK293 in WB, human lung tissue in IHC, NIH-3T3 and HeLa in IF.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu SNAIL

Target: SNAIL (SNAI1)

Andere Bezeichnung: SNAI1

Hintergrund: Rabbit Anti-SNAI1 Antibody, Snail Family Transcriptional Repressor 1, Snail Family Zinc Finger 1, Zinc Finger Protein SNAI1, Protein Snail Homolog 1, Protein Sna, SNAH, Snail 1 (Drosophila Homolog), Zinc Finger Protein, Snail Homolog 1 (Drosophila), Snail 1 Zinc Finger Protein, Snail 1 Homolog, Snail Homolog 1, DJ710H13.1, SLUGH2, SNAIL1, SNAIL, SNA,SNAI1 (Snail Family Transcriptional Repressor 1) is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. SNAI1 is involved in the induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. It binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. During EMT, SNAI1 is involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (By similarity). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). It associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, SNAI1 may also activate the CDKN2B promoter by itself. SNAI1 may play a role in Gastroesophageal Junction Adenocarcinoma and Fasciolopsiasis. Anti-SNAI1 Antibody is useful for researchers interested in Cytoskeleton remodeling Regulation of actin cytoskeleton by Rho GTPases and Kit receptor signaling pathways.

Gen-ID: 6615

NCBI Accession: NP_005976

UniProt: O95863

Pathways: Negative Regulation of intrinsic apoptotic Signaling