SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) Antikörper

Produktnummer ABIN7540581

Hersteller Produkt- Nr.: 600-401-a50

Der Kaninchen Polyklonal Anti--Antikörper wurde für ELISA, WB, IF, FACS, IHC und FM validiert. Er ist geeignet, in Proben von SARS Coronavirus (SARS-CoV) zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) Antikörper (ABIN7540581)

Target: SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N)

Reaktivität: SARS Coronavirus (SARS-CoV)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Produktdetails

Hersteller Produkt- Nr.: 600-401-a50

Hersteller: Rockland

Verwendungszweck: Sars Nucleocapsid Protein Antibody

Kreuzreaktivität (Details): Anti-SARS-CoV-1 (N) affinity purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein.

Aufreinigung: The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 (N) resin.

Sterilität: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus Nucleocapsid protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:500 - 1:2,000

Kommentare:

Suggested Applications: Other
Anti-SARS-CoV-1 purified antibody has been tested for use in ELISA, immunofluorescence, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46 kDa in size corresponding to SARS Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract. This antibody can be useful in ELISA and lateral flow format to detect virus in extracts from nasal and throat swabs and saliva. IF can be been useful to determine the presence or absence of virus entering cells especially when anti-viral drugs are applied. IHC studies can be performed on biopsies, detecting the coronavirus in lung, liver, bile duct, and placenta tissue. And may have the ability to neutralize the virus and thereby protect cells from the uptake of live virus. This antibody may be useful in flow cytometry studies.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

V'kovski, Gultom, Kelly, Steiner, Russeil, Mangeat, Cora, Pezoldt, Holwerda, Kratzel, Laloli, Wider, Portmann, Tran, Ebert, Stalder, Hartmann, Gardeux, Alpern, Deplancke, Thiel, Dijkman: "Disparate temperature-dependent virus-host dynamics for SARS-CoV-2 and SARS-CoV in the human respiratory epithelium." in: PLoS biology, Vol. 19, Issue 3, pp. e3001158, (2021) (PubMed).

Liu, Hu, Wang, Ren, Zhao, Ji, Zhu, Feng, Gong, Ju, Zhu, Cai, Lan, Guo, Xie, Dong, Zhu, Na, Wu, Lan, Xie, Wang, Yuan, Zhang, Ding: "Functional and genetic analysis of viral receptor ACE2 orthologs reveals a broad potential host range of SARS-CoV-2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 118, Issue 12, (2021) (PubMed).

Zhao, Ni, Gao, Wang, Yang, Wei, Lv, Liang, Zhang, Xu, Xie, Wang, Yuan, Liang, Zhang, Lin: "Recapitulation of SARS-CoV-2 infection and cholangiocyte damage with human liver ductal organoids." in: Protein & cell, Vol. 11, Issue 10, pp. 771-775, (2020) (PubMed).

Grant, Anderson, Williford, Alonzo, Glukhova, Boyle, Weigl, Nichols: "SARS-CoV-2 Coronavirus Nucleocapsid Antigen-Detecting Half-Strip Lateral Flow Assay Toward the Development of Point of Care Tests Using Commercially Available Reagents." in: Analytical chemistry, Vol. 92, Issue 16, pp. 11305-11309, (2020) (PubMed).

Antigendetails

Target: SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N)

Andere Bezeichnung: SARS-CoV Nucleocapsid

Substanzklasse: Viral Protein

Hintergrund: Rabbit anti-Sars Nucleocapsid Protein Antibody, rabbit anti-Sars-CoV Nucleocapsid (N) Protein Antibody, N antibody, N structural protein antibody, COVID19 antibody, Nucleocapsid protein antibody, Nucleoprotein antibody, SARS coronavirus N protein antibody, SARS CoV-2 antibody, SARSCoV2 antibody, Severe acute respiratory syndrome antibody, A50 Antibody, A50 SARS, COVID,The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid.  Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three-domain structure for the nucleocapsid protein. Anti-SARS-CoV Nucleocapsid (N) Protein Antibody is useful for researchers interested in viral research.

Gen-ID: 1489678, 30173007

UniProt: P59595