EEF1A2 Antikörper (C-Term)

Produktnummer ABIN7540568

Hersteller Produkt- Nr.: 600-101-ml6s

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch EEF1A2 in WB, ELISA, IHC, IF und FM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für EEF1A2 Antikörper (C-Term) (ABIN7540568)

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EEF1A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-EEF1A2 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 600-101-ml6s

Hersteller: Rockland

Verwendungszweck: EEF1A2 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human EEF1A2.

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-EEF1A2 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human EEF1A2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-EEF1A2 Antibody has been tested in WB, IF, and IHC.  Expect a band at ~50.5 kDa in western blot using appropriate lysates.  Positive control used: human skeletal muscle lysate, MCF-7 nuclear extract lysate in WB, MCF-7 cells in IF, and human skeletal muscle tissue in IHC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu EEF1A2

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Andere Bezeichnung: EEF1A2

Hintergrund: Goat Anti-EEF1A2 Antibody, Goat Anti-Eukaryotic Translation Elongation Factor 1 Alpha 2 Antibody, Eukaryotic Elongation Factor 1 A-2, EF-1-Alpha-2, Statin-S1, EEF1AL, STN 3, Elongation Factor 1-Alpha 2, Statin-Like, EEF1A-2, EIEE33, Statin, MRD38, EF1A, STNL, HS1,EEF1A2 (Eukaryotic Translation Elongation Factor 1 Alpha 2) protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. EEF1A2 may be associated with the development of ovarian cancer, along with Epileptic Encephalopathy, Early Infantile, 33 and Mental Retardation, Autosomal Dominant 38. Anti-EEF1A2 Antibody is useful for researchers interested in cancer research, translation factors, GTP binding, and GTPase activities.

Gen-ID: 1917

NCBI Accession: NP_001949

UniProt: Q05639