Doublecortin Antikörper (C-Term)

Produktnummer ABIN7540562

Hersteller Produkt- Nr.: 600-101-mh8s

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Doublecortin in WB, ELISA, IHC, IF und FM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Doublecortin Antikörper (C-Term) (ABIN7540562)

Target: Doublecortin (DCX)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Doublecortin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-Doublecortin Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 600-101-mh8s

Hersteller: Rockland

Verwendungszweck: Doublecortin Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human Neuronal migration protein doublecortin.

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-Doublecortin antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human doublecortin conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Doublecortin Antibody has been tested in WB, IF, and IHC.  Expect bands ~40.6 kDa in western blot using appropriate lysates.  Positive control used: PND2-6 brain cells in western blot, PND1 in immunofluorescence, and mouse dentate gyrus for immunohistochemistry.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Doublecortin

Target: Doublecortin (DCX)

Andere Bezeichnung: DCX

Hintergrund: Goat Anti-Doublecortin Antibody, Lissencephalin-X, Doublecortex,  Doublin, Lis-X, DBCN, LISX, Doublecortex Lissencephaly X-Linked (Doublecortin), Neuronal Migration Protein Doublecortin, SCLH, XLIS, DC,DCX (Neuronal Migration Protein Doublecortin) is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. In that way, it may participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. Doublecortin may be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Anti-Neuronal Migration Protein Doublecortin Antibody is useful for researcher interested in developmental biology, cytoskeletal signaling, neuroscience, and protein kinase binding.

Gen-ID: 1641

NCBI Accession: NP_000546

UniProt: O43602