ASCL1 Antikörper (Internal Region)

Produktnummer ABIN7540560

Hersteller Produkt- Nr.: 600-101-mh4s

Der Ziege Polyklonal Anti-ASCL1-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, ASCL1 in Proben von Maus zu detektieren.

Kurzübersicht für ASCL1 Antikörper (Internal Region) (ABIN7540560)

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ASCL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-ASCL1 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-101-mh4s

Hersteller: Rockland

Verwendungszweck: Mash1/Ascl1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against mouse Mash1/Ascl1.

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-Mash1/Ascl1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Mash1/Ascl1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Mash1/Ascl1 Antibody has been tested in Western Blot and Immunohistochemistry. Expect a band at ~24.7 kDa in western blot using appropriate lysates or tissues. Positive control used was mouse pancreas in WB, human thyroid papillary carcinoma tissue in IHC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Andere Bezeichnung: Ascl1

Hintergrund: Goat Anti-Ascl1 Antibody, Goat Anti-Mash1 Antibody, Achaete-scute homolog 1, ASH-1, mASH-1,Ascl1 (Achaete-scute homolog 1) is a transcription factor that plays a key role in neuronal differentiation, it acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. It directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Ascl1 plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. It is essential for the generation of olfactory and autonomic neurons. It acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Anti-Mash1/Ascl1 Antibody is useful for researchers interested in CCHS, SIDS, Dopaminergic Neurogenesis, and DNA binding.

Gen-ID: 17172

NCBI Accession: NP_032579

UniProt: Q02067

Pathways: Dopaminergic Neurogenesis